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BMCBI
2006
93views more  BMCBI 2006»
15 years 6 months ago
Evaluation of absolute quantitation by nonlinear regression in probe-based real-time PCR
Background: In real-time PCR data analysis, the cycle threshold (CT) method is currently the gold standard. This method is based on an assumption of equal PCR efficiency in all re...
Rasmus Goll, Trine Olsen, Guanglin Cui, Jon Florho...
BMCBI
2006
149views more  BMCBI 2006»
15 years 6 months ago
REtools: A laboratory program for restriction enzyme work: enzyme selection and reaction condition assistance
Background: Restriction enzymes are one of the everyday tools used in molecular biology. The continuously expanding panel of known restriction enzymes (several thousands) renders ...
Patrick Martin, Kim E. Boulukos, Philippe Pognonec
BMCBI
2006
175views more  BMCBI 2006»
15 years 6 months ago
Parameter estimation for stiff equations of biosystems using radial basis function networks
Background: The modeling of dynamic systems requires estimating kinetic parameters from experimentally measured time-courses. Conventional global optimization methods used for par...
Yoshiya Matsubara, Shinichi Kikuchi, Masahiro Sugi...
BMCBI
2006
143views more  BMCBI 2006»
15 years 6 months ago
IsoSVM - Distinguishing isoforms and paralogs on the protein level
Background: Recent progress in cDNA and EST sequencing is yielding a deluge of sequence data. Like database search results and proteome databases, this data gives rise to inferred...
Michael Spitzer, Stefan Lorkowski, Paul Cullen, Al...
BMCBI
2006
160views more  BMCBI 2006»
15 years 6 months ago
Gene prediction in eukaryotes with a generalized hidden Markov model that uses hints from external sources
Background: In order to improve gene prediction, extrinsic evidence on the gene structure can be collected from various sources of information such as genome-genome comparisons an...
Mario Stanke, Oliver Schöffmann, Burkhard Mor...